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Concentrating total RNA....


Bailo

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What kind of RNA?

 

This site might help... protocol-online

:) Already checked there. It's total RNA for the synthesis of microarray probes, so in effect it's mRNA. That's where I got ethanol precipitation from ;)

 

How will you inhibit cellular mitosis?

Bacteria mate, no mitosis here.

 

Burn it, then behead someone.

My supervisor is right in line for that.

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:) Already checked there. It's total RNA for the synthesis of microarray probes, so in effect it's mRNA. That's where I got ethanol precipitation from ;)

There's a few kits out there you can use, but I think they generally use ethanol precipitation anyway...
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There's a few kits out there you can use, but I think they generally use ethanol precipitation anyway...

Yeah, and I don't want to wait for a kit to arrive. Sodium acetate and ethanol, here we come! Better get 100% back from this :hmm:

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:) Already checked there. It's total RNA for the synthesis of microarray probes, so in effect it's mRNA. That's where I got ethanol precipitation from ;)

Bacteria mate, no mitosis here.

My supervisor is right in line for that.

Ah - petrie cultures or using a controlled B/SGM?

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Ah - petrie cultures or using a controlled B/SGM?

No idea what the second is ;) It's from a MOPS buffered minimal broth.

 

A thread on mixing booze into whank. Sick puppies.

I personally wouldn't drink 99% ethanol. Burns too much on the way down.

 

98% though....

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No idea what the second is ;) It's from a MOPS buffered minimal broth.

I personally wouldn't drink 99% ethanol. Burns too much on the way down.

 

98% though....

Ah - ok gone almost as far as I can remember now! A controlled B/SGM = Bacterial/Sporic Generating Medium.

 

Good luck! (Unless you work at Huntingdon Life Sciences) ;)

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